Tuesday, August 22, 2006

Gene function and pathway analysis sites

This is a series of links which are helpful in analysing gene funtions by either entering a single Gene ID, or by entering a bunch of genes (gene lists from microarray analysis for eg). Just click the Blue word in each description and it will take you there.

If you find another site that you have found helpful, please let me know and I will add it to this list. Hope this helps though!
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This is a link to the SOURCE website which allows you to input your gene (as a GenBank Accession number for eg) and get the gene name, symbol, ontologies, function, and several other potentially useful information. It is possible to do a single gene search, or a batch analysis. It is also possible to download your results as a tab-delimited .txt file.

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This is a link to the DAVID Bioinformatics website, which allows you to do similar things as the SOURCE website such as functional annotation. This site has several other useful features such as producing "Enrichment" scores, which, to my understanding, is an analysis of the likely-hood of a group of genes being found together. Or conversely, it gives you a numerical interpretation as to the likely-hood that these genes would come up together in a list at random. The results that this program produces all have links as well as many other useful statistical analysis. Well worth a look. (Performs an EASE analysis)


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This is another DAVID analysis website which allows you to post in gene lists from microarray experiments (or other experiment producing lists of genes) and results in sorting them into different functional categories based on their GO (Gene Ontology) Terms. It produces Annotation charts, GO Charts, Kegg Charts, Domain Charts, and provides access to EASE online (once you submit a gene list). You can also download EASE to run on your own computer from this site by following the links. I gathered alot of useful information from this website, and recommend it.

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The Gene Expression Omnibus (GEO) website which is maintained by NCBI. I have not used it, but it comes with high expectations. If you have used it, please drop a line and I'll incorporate it into this short profile.

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This is the KEGG Pathway Database website, which is part of the Kyoto Encyclopedia of Genes and Genomes, and is a great database allowing you visualize heaps of different pathways. It's also interactive, so you can find a pathway (for eg) and then click on a protein or enzyme of your choice, and then get sequence info, what other pathways is it linked with, and other info depending on the type of protein it is. Simply put, I like this site.

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This is a link to the "Microarray Analysis Resources" website, which has a much more comprehensive list of microarray resources, including general databases, Analysis Tools, Functional Annotation (GO tools), Pathway Analysis and a few others. A good resource to help you analyze your data in general.

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